Standard Insemination
Stimulation of the ovaries: Based on pre-IVF scans & blood tests a stimulation regime is decided to stimulate the ovaries to produce eggs. Regular ultrasounds reports and some blood tests reports are used to continually monitor ovary’s response to this stimulation.
HOPE IVF SMART SCIENCE: The egg stimulation mimics your body’s natural processes. It doesn’t affect future egg supply or lead to premature menopause
Step 1: Egg collection: Once the eggs are ready in the ovary then with a small surgical procedure the eggs are aspirated out, you would be sedated so that you don’t feel anything. Same day your husband’s semen is also collected, depending on the present and past status the eggs/sperms are processed accordingly.
Step 2: Conventional Insemination: If the embryologist has decided for conventional Insemination as the mode of fertilisation, then the sperms are carefully processed and a calculated concentration of the sperm is aliquoted around the eggs in a specific dish, medium micro droplet and environment. By this process the eggs are fertilised and thus the eggs are activated to grow further ahead in coming days.
Step 3: Embryo growth: For two to seven days your embryos are created by your eggs & sperms and are further grown to advanced stages. This growth is directly related to the quantity and quality of the eggs and sperms used and it differs from couple to couple. This growth sometimes is done for three days and sometimes for five to six days too.
Step 4: Embryo Transfer: Once the embryo growth has taken place then the embryologist decides which embryo should be send back in your womb and which embryos can be cryopreserved.
Step 5: Blood test: This is a blood test often done after two week of your embryo transfer, to confirm healthy implantation of the embryo in you.
ICSI : Intra Cytoplasmic Sperm Injection
This line of treatment is mostly used for couples with male factor infertility like low sperm count, no sperm count, low sperm morphology and fertilisation failure by conventional insemination.
Stimulation of the woman: Based on pre-IVF scans & blood tests a stimulation regime is decided to stimulate the ovaries to produce eggs. Regular ultrasounds scan reports and some blood tests reports are used to continually monitor ovary’s response to this stimulation.
Step 1: Egg collection: Once the eggs are ready in the ovary then by a small surgical procedure the eggs are aspirated out, you will be sedated so you won’t feel anything. Same day your husband’s semen is also collected and depending on the present and past status both the eggs and sperm are processed accordingly.
Step 2: Intra Cytoplasmic Sperm Injection: If the embryologist has decided for ICSI as the mode of fertilisation, then after processing the eggs & sperms, a single sperm based on its motility and morphology is selected & injected into the egg to fertilise it by a microsurgical procedure with help of micromanipulators.
Step 3: Embryo growth: For two to seven days your embryos created by your eggs & sperms are grown and taken care of. This growth is directly related to the quantity and quality of the eggs and sperms used and it differs from couple to couple. This growth sometimes is done for three days and sometimes for five to six days too.
Step 4: Embryo transfer: Once the embryo growth has taken place then the embryologist decides which embryo should be send back in your womb and which embryos can be cryopreserved.
Step 5: Blood test: This is a blood test often done after two week of your embryo transfer, to confirm healthy implantation of the embryo in you
Blastocyst culture
The egg once fertilised by sperm is called embryo and this embryo starts growing from its single cell stage to multiple cell stage within a fixed time duration.
The growth that takes place approximately till 30 hours is called cleavage stage growth, around 72 hours embryo reaches its morulla stage and 5th day it is in the stage of blastocyst with its largest size, a fluid filled cavity in between, all cells pushed to the periphery and around 200-300 number of cells.
Blastocyst stage embryo transfer increases pregnancy chances however not every embryo is destined to reach the blastocyst stage. Other than the obvious egg / sperm quality the embryology laboratory and high skills of an embryologist are vital in helping an embryo to reach its blastocyst stage.
This growth beyond day three is also often termed as the extended growth of the embryo and since it increases chances of your pregnancy we at HOPE have specific protocols and dedicated separate instruments that enhance an embryo’s capability to reach its blastocyst stage.